Effetti dell'aloe sulla leucemia negli uomini
Induction of apoptosis in human leukaemic cell lines K562,
HL60 and U937 by diethylhexylphthalate isolated from Aloe vera Linne.
Lee KH, Hong HS, Lee CH, Kim CH. Animal Resource Research Center, Konkuk
University, Seoul, Korea.
We investigated the effect of diethylhexylphthalate (DEHP) from Aloe vera Linne
on the apoptosis of human leukaemic cell lines K562, HL60 and U937 to examine
its pharmacological activity. At a level of 10 microg mL(-1) DEHP a significant
anti-leukaemic effect was observed for all three cell lines, as measured by
clonogenic assay. After treatment with 10 microg mL(-1) DEHP for 4 h, agarose
gel electrophoresis and flow cytometric analysis confirmed the occurrence of
apoptosis. These results indicate that DEHP isolated from Aloe vera Linne has a
potent antileukaemic effect, and thus represents a new type of pharmacological
activity with respect to human leukaemic cells. PMID: 11007077
Anti-leukaemic and anti-mutagenic effects
of di(2-ethylhexyl)phthalate isolated from Aloe vera Linne.
Lee KH, Kim JH, Lim DS, Kim CH. Animal Resource Research Center, Konkuk
University, Seoul, Korea.
Extracts of Aloe vera Linne have been found to exhibit cytotoxicity against
human tumour cell lines. This study examines the anti-tumour effects of
di(2-ethylhexyl)phthalate (DEHP) isolated from Aloe vera Linne, in human and
animal cell lines. Its anti-mutagenic effects were examined using Salmonella
typhimurium TA98 and TA100 strains. Growth inhibition was specifically exerted
by DEHP against three leukaemic cell lines at concentrations below 100 microg
mL(-1). At 100 microg mL(-1) DEHP, K562, HL60 and U937 leukaemic cell lines
showed growth inhibition of 95, 97 and 95%, respectively. DEHP exhibited an
inhibitory activity of 74, 83 and 81%, respectively, in K562, HL60 and U937 cell
lines at a concentration of 10 microg mL(-1). At a concentration of 1 microg
mL(-1), DEHP exerted an inhibitory activity of 50, 51 and 52%, respectively, in
K562, HL60 and U937. In a normal cell line, MDBK, DEHP exerted 30% growth
inhibition at a concentration of 100 microg mL(-1), and showed no inhibitory
activity at concentrations below 50 microg mL(-1). It was found that DEHP
exerted anti-mutagenic activity in the Salmonella mutation assay. The number of
mutant colonies of Salmonella typhimurium strain TA98 upon exposure to AF-2 (0.2
microg/plate) decreased in a concentration-dependent manner in the presence of
different DEHP concentrations (decreasing to 90.4, 83.9, 75.4, 69.6 and 46.9%,
respectively, for DEHP concentrations of 100, 50, 10, 5 and 1 microg/plate). In
the case of Salmonella typhimurium strain TA100, DEHP reduced AF-2-induced
mutagenicity at 1, 5, 10, 50 and 100 microg/plate to 57.4, 77.5, 80.0, 89.0 and
91.5%, respectively. The isolated compound from Aloe vera Linne, DEHP, was
considered to be the active principle responsible for anti-leukaemic and
anti-mutagenic effects in-vitro. PMID: 10864149
Aloe-emodin is a new type of anticancer
agent with selective activity against neuroectodermal tumors.
Pecere T, Gazzola MV, Mucignat C, Parolin C, Vecchia FD, Cavaggioni A, Basso
G, Diaspro A, Salvato B, Carli M, Palu G. Department of Histology,
Microbiology, and Medical Biotechnologies, Medical School, University of Padova,
Here we report that aloe-emodin (AE), a hydroxyanthraquinone present in Aloe
vera leaves, has a specific in vitro and in vivo antineuroectodermal tumor
activity. The growth of human neuroectodermal tumors is inhibited in mice with
severe combined immunodeficiency without any appreciable toxic effects on the
animals. The compound does not inhibit the proliferation of normal fibroblasts
nor that of hemopoietic progenitor cells. The cytotoxicity mechanism consists of
the induction of apoptosis, whereas the selectivity against neuroectodermal
tumor cells is founded on a specific energy-dependent pathway of drug
incorporation. Taking into account its unique cytotoxicity profile and mode of
action, AE might represent a conceptually new lead antitumor drug. PMID:
Studio degli effetti dell'aloe contro
tumori della pelle.
Adverse and beneficial effects of plant
extracts on skin and skin disorders.
Mantle D, Gok MA, Lennard TW. Department of Surgery, Medical School,
University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU.
Plants are of relevance to dermatology for both their adverse and beneficial
effects on skin and skin disorders respectively. Virtually all cultures
worldwide have relied historically, or continue to rely on medicinal plants for
primary health care. Approximately one-third of all traditional medicines are
for treatment of wounds or skin disorders, compared to only 1-3% of modern drugs.
The use of such medicinal plant extracts for the treatment of skin disorders
arguably has been based largely on historical/anecdotal evidence, since there
has been relatively little data available in the scientific literature,
particularly with regard to the efficacy of plant extracts in controlled
clinical trials. In this article therefore, adverse and beneficial aspects of
medicinal plants relating to skin and skin disorders have been reviewed, based
on recently available information from the peer-reviewed scientific literature.
Beneficial aspects of medicinal plants on skin include: healing of wounds and
burn injuries (especially Aloe vera); antifungal, antiviral, antibacterial and
acaricidal activity against skin infections such as acne, herpes and scabies (especially
tea tree (Melaleuca alternifolia) oil); activity against inflammatory/immune
disorders affecting skin (e.g. psoriasis); and anti-tumour promoting activity
against skin cancer (identified using chemically-induced two-stage
carcinogenesis in mice). Adverse effects of plants on skin reviewed include:
irritant contact dermatitis caused mechanically (spines, irritant hairs) or by
irritant chemicals in plant sap (especially members of the Ranunculaceae,
Euphorbiaceae and Compositae plant families); phytophotodermatitis resulting
from skin contamination by plants containing furocoumarins, and subsequent
exposure to UV light (notably members of the Umbelliferae and Rutaceae plant
families); and immediate (type I) or delayed hypersensitivity contact reactions
mediated by the immune system in individuals sensitized to plants or plant
products (e.g. peanut allergy, poison ivy (Toxicodendron) poisoning). PMID: 11482001
Effetti dell'aloe contro i tumori
Acemannan purified from Aloe vera induces
phenotypic and functional maturation of immature dendritic cells.
Lee JK, Lee MK, Yun YP, Kim Y, Kim JS, Kim YS, Kim K, Han SS, Lee CK.
College of Pharmacy, Chungbuk National University, Cheongju 361-763, South Korea.
Acemannan, a major carbohydrate fraction of Aloe vera gel, has been known to
have antiviral and antitumoral activities in vivo through activation of immune
responses. The present study was set out to define the immunomodulatory activity
of acemannan on dendritic cells (DCs), which are the most important accessory
cells for the initiation of primary immune responses. Immature DCs were
generated from mouse bone marrow (BM) cells by culturing in a medium
supplemented with GM-CSF and IL-4, and then stimulated with acemannan, sulfated
acemannan, and LPS, respectively. The resultant DCs were examined for phenotypic
and functional properties. Phenotypic analysis for the expression of class II
MHC molecules and major co-stimulatory molecules such as B7-1, B7-2, CD40 and
CD54 confirmed that acemannan could induce maturation of immature DCs.
Functional maturation of immature DCs was supported by increased allogeneic
mixed lymphocyte reaction (MLR) and IL-12 production. The
differentiation-inducing activity of acemannan was almost completely abolished
by chemical sulfation. Based on these results, we propose that the adjuvant
activity of acemannan is at least in part due to its capacity to promote
differentiation of immature DCs. PMID: 11460308
Decreased mortality of Norman murine
sarcoma in mice treated with the immunomodulator, Acemannan.
Peng SY, Norman J, Curtin G, Corrier D, McDaniel HR, Busbee D.
Department of Anatomy, College of Veterinary Medicine,
Texas A & M University, College Station 77843.
An extract from the parenchyma of Aloe barbadensis Miller shown to contain long
chain polydispersed beta (1,4)-linked mannan polymers with random O-acetyl
groups (acemannan, Carrisyn) was found to initiate the phagocyte production of
monokines that supported antibody dependent cellular cytotoxicity and stimulated
blastogenesis in thymocytes. Acemannan, in both enriched and highly purified
forms, was administered intraperitoneally to female CFW mice into which murine
sarcoma cells had been subcutaneously implanted. The rapidly growing, highly
malignant and invasive sarcoma grew in 100% of implanted control animals,
resulting in mortality in 20 to 46 days, dependent on the number of cells
implanted. Approximately 40% of animals treated with acemannan at the time of
tumor cell implantation (1.5 x 10(6) cells) survived. Tumors in
acemannan-treated animals exhibited vascular congestion, edema,
polymorphonuclear leukocyte infiltration, and central necrosing foci with
hemorrhage and peripheral fibrosis. The data indicate that in vivo treatment of
peritoneal macrophages stimulates the macrophage production of monokines,
including interleukin-1 and tumor necrosis factor. The data further indicate
that sarcomas in animals treated i.p. with acemannan at the time of tumor cell
implantation were infiltrated by immune system cells, became necrotic, and
regressed. The combined data suggest that acemannan-stimulated synthesis of
monokines resulted in the initiation of immune attack, necrosis, and regression
of implanted sarcomas in mice. PMID: 1910624
Tumori dei polmoni
The therapeutic potential of Aloe Vera in
Corsi MM, Bertelli AA, Gaja G, Fulgenzi A, Ferrero ME. Institute of
General Pathology, Medical Faculty, University of Milan, Italy.
Aloe Vera has been claimed to contain several important therapeutic properties,
including anticancer effects. The effect of Aloe Vera administration was studied
on a pleural tumor in rat. Growth of Yoshida AH-130 ascite hepatoma cells
injected (2 x 10(5) in 0.1 ml) into pleura of male inbred Fisher rats was
evaluated at different times (7th and 14th days). Data show that the use of Aloe
Vera proved a therapeutic method, and that the present experimental model could
be useful in the study of other therapeutics treatments in vivo.